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Cells were transfected in parallel with StealthTM Negative Control.
In brief, Dam-fusion and Dam-only expression vectors were transfected in parallel into separate dishes of Kc cells by electroporation.
Cells were transfected in parallel with Rev-dependent or PRE-dependent Gag, or with G2A mutant HIV-1 Gag expression plasmids.
To investigate whether the nuclear translocation is dependent on the activation of PKCδ, SH-SY-5Y cells were transfected, in parallel experiments, with PKCδ and with Dominant Negative PKCδ (PKCδ-DN) plasmid, the resulting overexpressing cells then being treated either with BSO or etoposide.
For each experiment, three 35 mm dishes were transfected in parallel.
APE1 wildtype cDNA expression vector and a vector only control were transfected in parallel into RPMI-8226 cells.
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wt-N6/C6 was transfected in parallel as a positive control.
An empty vector was transfected in parallel with pCMV-S100A4 and was used as negative control.
For a positive control, the I-SceI expression plasmid was transfected in parallel.
Luciferase reporter vector with PDCD4 mutant target was transfected in parallel as control.
As a control, the negative control siRNA (Ambion) was transfected in parallel.
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