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cDNAs were linearized and were transcribed to cRNA in vitro using the mMessage mMachine kit (Ambion, Austin, TX, USA).
Correspondingly, a great amount of RNA strands complementary to MB and partial primers were transcribed to initiate new cyclic reactions of MB switch, ligation, and transcription.
...all of the handwritten notecards (representing mainly talks to students on campus) were transcribed to the typewritten forms so that future researchers can more easily work with the outlines on the notecards.
DNA fragments corresponding to Cas9 and the chimeric RNA were transcribed to RNAs in vitro by the T7 RNA polymerase and the Cas9 RNA was further modified by adding a translation-required cap at the 5′-end and a poly-A tail at the 3′-end to make it resemble an authentic mRNA.
Afterwards, the interviews were transcribed to analyze the collected material.
All interviews were transcribed to word-processed files as originally recorded, then verified by a separate proofreader.
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The Agency Directive was transcribed to UK law by the Commercial Agents Regulations 1993.
The amplified region was cloned and the plasmid was transcribed to generate calibration standards for quantification.
RNA was transcribed to contain the same transducer sequence as that which is expressed as circular RNA by Tornado.
One such tool is the microarray, or expression chip, which can display which of a cell's genes are switched on and being transcribed to make protein.
Total RNA was extracted from cells using Trizol (Invitrogen) and was transcribed to cDNA by using a high capacity cDNA reverse transcription kit (Applied Biosystems, CA, USA).
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