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The field observations were then supplemented with a series of controlled microcosm experiments designed to test those hypotheses.
A commercial cellulase cocktail, the CBHI from Trichoderma reesei (TrCBHI) and the CBHI CD from T. reesei (CDCBHI) were then supplemented with the purified CBM and the activity enhancement on the filter paper hydrolysis was analyzed.
Cells were then supplemented with no copper, 10 µM or 50 µM at time of transfection.
Mice were deprived of circulating androgen by bilaterally castration and were then supplemented with exogenous testosterone propionate for one week.
Upon transfection, cells were allowed to grow for 16 24 hours, and were then supplemented with 10% FBS for 24 hours followed by the treatment.
The media were then supplemented with either 100 µM simvastatin or buffer and the cells were further grown with shaking for two hours at 30°C.
Similar(26)
This is then supplemented with physiotherapy and regular monitoring.
For the islanding operation, the deficiency of steam generation is then supplemented by boilers.
This mechanism was then supplemented with the reactions involving NO formation, followed by validations in both homogeneous and diffusive systems.
The perturbed system is then supplemented by an appropriate compatibility condition which arises from the properties of the gradient operator.
The study is then supplemented by experimental tests developed in the Laboratory of Experimental Dynamics at the University of Palermo, Italy.
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