Exact(39)
The products were then reverse transcribed into cDNA again, using random hexamer primers.
Samples were then reverse transcribed with Superscript III (Invitrogen) and aadUTP was incorporated.
The isolated oxidized mRNAs were then reverse transcribed to cDNAs with DIG-labeled dUTPs to facilitate analysis by Southern blotting.
102 to 106 RNA copies of each transcript were then reverse transcribed and amplified as described above.
Equal amounts of RNA from each sample were then reverse transcribed to cDNA by using a Superscript first-strand synthesis kit (Invitrogen).
One hundred and fifty ng of total RNA were then reverse transcribed into cDNA using the LightCycler® Transcriptor First Strand cDNA Synthesis Kit (Roche, Basel, Switzerland).
Similar(21)
The crosslinks were then reversed at 65 °C for 3 h.
The cross-links were then reversed, and DNA was purified following the manufacturer's instructions.
Some of those suspicions turned into diagnoses, and some of those diagnoses were then reversed.
The roles were then reversed as it was Cardiff's turn to respond to falling behind.
Aliquots (∼1 µg) of the total RNA extracts were then reverse-transcribed into cDNA using a First Strand cDNA Synthesis Kit (Amersham Pharmacia Biotech, Oakville, ON, Canada).
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