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Animals were then restrained in small metal tube with strips of tape on the abdomen and behind the head.
All three NCS groups were then restrained with tight positional restraints throughout the refinement in Refmac 5.02, but the heavy chain of AbD1556 molecule 1 (chain H) was excluded from NCS refinement as the strands β6 and β7 of the constant heavy (CH) domains were shifted by about 1.4 to 1.8 Å compared to the other three CH domains due to differences in the crystal lattice contacts (see Fig. 1c).
Honey bees were then restrained in small polyacryl holders using strips of duct tape.
Briefly, anesthesia was induced with 4-5% isoflurane in an anesthetic chamber; mice were then restrained supine on a heated platform.
They were then restrained in a stereotactic apparatus (Stoelting) and implanted bilaterally with 10 ± 0.02 mm, 23gauge stainless steel guide cannulae above the IC.
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The pitch invader was then restrained by two Oldham players and led away.
She is then restrained.
He is then restrained for a period of up to three hours so he does not purge his meal.
ADF is then restrained by the muscle's tension.
Each cow was then restrained in a hydraulic chute with overturning capability.
The sheep are then restrained in a sling cage and the ventral aspect of the neck is shaved to facilitate vascular access.
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