Ammonium and phosphate were then recovered as struvite during crystallization.
The swollen samples were then recovered with 100-mesh sieve.
The vitrified seeds were then recovered and sterilised as described above.
Attached cells were then recovered into 10 ml of PPB by surface scraping.
Stx1 and Stx2 were then recovered from the treated spots, and subjected to various analyses.
After 96 h of the culture, the HAECs were detached from the microchips by decreasing the temperature and were then recovered from the microchannels.
Weathered asphalt binders were then recovered from the weathered mixtures by the Abson method, consequently separated into four common components, i.e., saturate, aromatic, resin and asphaltene (SARA).
The cells were then recovered in mTeSR1 medium supplemented with 10 mmol/L Rho-associated kinase (ROCK) inhibitor Y-27632 (10 mmol/L, Sigma) after electroporation.
The beads were then recovered by filtering through a filter paper (Whatman no. 42), spread on petri dish, dried for 2 days at room temperature and stored in an air-tight container for further use.
(G) Human gastric cancer cell line SGC7901 cells stably overexpressing SENP3 or vector were inoculated into nude mice to form tumor xenografts, and the tumors were then recovered, minced into small pieces and implant beneath the serosa of the stomach of other mice to grow for eight weeks (upper).
Cell fractions were then recovered and spun at 1150 rpm for 12 min at 4°C.
