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The rods were then post cured for 1 day at 70 °C to complete the polymerization process and relieve any mechanical stresses present.
The halves were then post fixed in osmium tetraoxide in O.2 M sodium cacodylate buffer, dehydrated in graded series of ethanol (70%100%00%) and embedded in epoxy resin.
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The children were then post-tested on the trained task and three additional inductive reasoning measures.
The samples were then post-fixed and stained with 1% osmium tetroxide followed by 1% uranyl acetate.
The iCLEM program was started in 2007 by Clem Fortman and James Carothers who were then post-doctoral researchers at JBEI and SynBERC.
Some of the samples were then post-oxidized at 700 °C for 15 and 60 min, within a O2 plasma of 9.5 Pa.
The cells were then post-fixed with 1% OsO4 in 0.2 M phosphate buffer (pH 7.2) for 2 h at 4°C.
The samples were then post-fixed by 1% OsO4 for 2 h, followed by dehydration in a graded ethanol series (30%, 50 %, 70 %, 90 % and 100
Image data were then post-processed with a Gaussian MAF (FWHM: ΓM = 7 mm) and a Gaussian BF (spatial domain: ΓS = 9 mm, intensity domain: ΓI = 2.5 SUV), respectively.
For each experiment, 1,500 nPIV image pairs of 256 × 80 pixels were acquired with an inter-frame time delay of 0.6 ms. The images were then post-processed using a standard FFT-based cross correlation program that uses a 3D Gaussian peak finding algorithm based on a Gaussian surface fit to determine the tracer particles' displacements[25].
The cells were then post-fixed in 1% osmium tetroxide for 2 h at 4°C, washed again with PBS, dehydrated through a series of alcohol concentrations (20, 30, 40, 50, 60, 70%), and followed by further dehydration 90, 96, 100% and dry alcohol).
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