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Cells were seeded onto synthetic scaffolds, which were then maintained in a bioreactor.
The plates were then maintained in an incubator at a constant temperature of 44 °C for 24 h.
This approach was also applied to transfer HUVECs to 3D geometrically defined vascular-like structures in hydrogels, which were then maintained in perfusion culture for 15 days.
Samples were then maintained for 120 s in a diluted 2% hydrofluoric acid (HF) solution to remove small fragments, roughen diatoms surface, and promote Au nucleation.
Cultures were then maintained under normal growth conditions.
All offspring were then maintained on control lighting conditions up to the age of 12 months.
Recombinant plasmids were then maintained and propagated in DH5α E. coli.
Detector voltages were then maintained for each staining condition across all sections.
These G418 and metal concentrations were then maintained continuously, providing sustained selection pressure and promoter induction.
The cells were then maintained for maturation and differentiation in B27-supplemented Neurobasal culture medium.
Cells were then maintained in defined medium in the presence of 20 ng/ml of FGF2 from day 8 [7].
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were then updated
were then sustained
were therefore maintained
were then adhered
were thereafter maintained
were thus maintained
were then ascertained
were then administered
were then retained
have subsequently maintained
have subsequently been maintained
were subsequently maintained
were originally maintained
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CEO of Professional Science Editing for Scientists @ prosciediting.com