Exact(40)
The TEM grids were then blotted and washed twice in distilled H2O before staining with uranyl acetate 2% for 2 min for visualization by TEM (JEOL JEM-1010).
The plantlets were then blotted dry on paper towel and their fresh weights, number of shoots and roots, length of roots and shoots were recorded.
The membranes were then blotted with specific primary antibodies for 1 h and washed 3 times for 5 min with TBS containing 0.1%Tween-20Tween-20 followed by incubation with secondary antibodies conjugated to horseradish peroxidase for 45 min and washed 5 times for 5 min with TBS containing 0.1%Tween-20Tween-20at room temperature.
Proteins were then blotted.
The separated RNAs were then blotted overnight to Hybond-N+ membrane (AP Biotech) by capillary transfer using 10×SSC [60].
The proteins were then blotted onto a Hybond-P membrane (Amersham Pharmacia Biotech) by using SemiPhor Semi-Dry Transfer Unit (Amersham Pharmacia Biotech).
Similar(20)
The heart was then blotted and its wet mass measured.
The staining solution was then blotted dry and the grids were stored.
The precipitate was then blotted with a 1 250 dilution of anti-rabbit phosphoserine (Zymed Laboratories, Inc., San Francisco, CA).
The gel was then blotted to a positively charged nylon membranes by the alkali transfer procedure.
The whole blood is then blotted onto a DBS collection card.
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