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The mice were then anesthetized.
Fish were then anesthetized and regenerates were collected for further analysis.
These mice were then anesthetized and fixed by perfusion with 4% buffered paraformaldehyde.
Animals were then anesthetized at 2 h after reperfusion (i.e., 1.5 h after the completion of treatment) or sham operation for collection of blood and tissue samples.
Hamsters were then anesthetized with sodium pentobarbital (200 mg/kg) and perfused transcardially with 150 ml of 0.9% saline followed by 300 ml of 4% paraformaldehyde in 0.1 M PBS (pH 7.4) 24 hours after the last BrdU injection.
The nematodes were allowed to ingest the microspheres for 10 additional min (15 min total) and were then anesthetized using 5 µL of sodium azide (10 mM, final concentration), inhibiting further bead ingestion.
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More usually performed on animals, the creature is given time to get used to the mirror and is then anesthetized and marked on the face with odourless, non-tactile dye.
Dechorionated embryos at 48 hpf are then anesthetized and prepared for cardinal vein injection as described above.
The right hindlimb of all the animals were shaved, and each rat was then anesthetized with isoflurane (aspiration rate, 450 mL/min; concentration, 2.0%).
Each mouse was then anesthetized, and ∼100 female Anopheles stephensi mosquitoes were allowed to feed for 20 min. Unfed mosquitoes were removed the following day.
The animals were retested after 26 days for further three days (2 trials each day) and were then (d40) anesthetized and transcardially perfused.
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