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Control straws were thawed and inseminated directly.
Snow samples were thawed overnight at 21 °C.
In the operating room, these strips were thawed in a small tub of saline.
Cryopreserved differentiated HepaRG cells (Biopredic International, France) were thawed according to the supplier's instructions.
To prepare for antibody staining, slides were thawed then rehydrated in PBS.
Cryovials were thawed quickly at 37 °C until no ice was observed in the solution.
Cryopreserved PHH (Bioreclamation IVT, USA) were thawed according to the supplier's instructions.
Human BM and blood were thawed from cyroperservation into PBS (Lonza).
Vials from each treatment group were thawed after 2, 5, 10 weeks and 58 weeks.
Aliquots of whole blood were thawed on ice and vortexed briefly to ensure homogeneity.
Working solutions were thawed for use immediately beforehand at room temperature.
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