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Cryostat-cut sections were thaw mounted onto Probe-on plus slides (Electron Microscopy Sciences, Hatfield, PA).
Cryo-sections were thaw mounted onto gelatin-coated slides and stored at -20°C.
The sections were thaw mounted onto glass slides prior to further processing (Fig. 2 A, B ).
Significant predictors of the number of AIV cases per county in wild birds were thaw date, the % of harvested cropland in the county, and minimum temperature (Table 3).
The sections were thaw mounted onto Super Frost/Plus object glasses (Menzel-Gläzer, Braunschweig, Germany) and stored at −20°C prior to use.
Frozen tissue sections were thaw mounted on microscope slides or placed into eppendorf tubes (for the homogenization procedure) and stored in a desiccator until needed.
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Tissue sections were thaw-mounted on glass slides and stored at −80 °C.
Frozen slices were thaw-mounted onto superfrost slides (Menzel-Gläser SUPERFROST plus microscopy slides, Thermo Scientific, Germany).
The tissue sections were thaw-mounted onto Superfrost Plus (Fisherbrand 12-550-15 (Fisherbrand, Leicestershire, UK)) microscope slides, allowed to air dry for 10 to 15 min, and stored at −80°C.
Coronal brain sections (20 μm) were thaw-mounted onto microscope slides, air dried, and apposed to an imaging plate (Fuji Imaging Plate BAS-TR2025, Fuji Photo FiLtd.o., Ltd., Minato-ku, Tokyo, Japan) for 4 h.
Frozen brains of young (10 12 weeks of age; 300 350 g body weight) male Sprague-Dawley rats (Harlan, Netherlands) were cut at −12 °C into sagittal slices 20 μm thick using a Leica microtome, and the slices were thaw-mounted on Superfrost (70 × 22 mm, Fischer) adhesive slides.
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