Sentence examples for were successively treated from inspiring English sources

DAPI staining was performed after [21] with the following modifications: after fixation in 4% formaldehyde, the wings were successively treated at 17°C with chloroform for 1 h, heptane for 2 h, and 1× PBS/0.2% Tween 20 for 3 h, prior to the overnight DAPI (1∶10000, Sigma) staining at 17°C.

The mice were successively treated with the same dose of Bifidobacterium infantis every 3 days, which lasted for 21 days.

In these studies, obese type 2 diabetic patients were successively treated by CSII and MDI for periods of 12 and 18 weeks, respectively (10, 11).

Cells were successively treated with 2 mL diluted FACS lysing solution (BD, Amsterdam, The Netherlands) for 10 minutes and then washed twice in wash buffer and immediately analyzed by flow cytometry.

Afterwards, cells were successively treated with 2 ml diluted FACS lysing solution (BD Biosciences) for 10 minutes and then washed twice in wash-buffer and immediately analyzed by flow cytometry.

The cells were successively treated with each rFab fragment (10 μg/mL in 1% BSA PBS), and 1 200 diluted FITC-labeled rabbit anti-mouse IgG F ab' 2 (Jackson ImmunoResearch Laboratories, West Grove, PA, USA).

After filtration, the residue was successively treated with ethyl acetate and n-butanol.

Accordingly, titanium was successively treated with magnesium and silver plasma immersion ion implantation & deposition (Mg Ag PIII&D).

After filtration, the aqueous solution was successively treated with ethyl acetate and n-butanol, and then their extracts were concentrated to dryness [12, 13].

The patient has been successively treated with vindesine/dacarbazine, vindesine/dacarbazine, and dacarbazine/interferon α.

One patient was successively treated in both legs, with an interval of 6 months between the two interventions.