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The two holo-ApcAs were successfully reconstituted in E. coli and purified by metal affinity chromatography.
More than 100 mice were successfully reconstituted and intraperitoneally inoculated with 100 µl of inoculum.
These data suggest that TE proteins were successfully reconstituted into vesicles.
Three months after transplantation, complete blood count analysis of peripheral blood of recipient mice showed that various blood cell lineages were successfully reconstituted by both the WT and Smurf2-deficient donors (Fig. S5B).
Complete blood count analysis of recipient peripheral blood 3 months after transplantation showed that various blood cell lineages were successfully reconstituted by both the WT and Smurf2-deficient donors (Fig. S5C).
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These assemblies could also be successfully reconstituted after freeze-drying.
The 72-residue polypeptide embedding the transmembrane helices 3 and 4 of the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) (residues 194 241) requires a high salt concentration in order to be successfully reconstituted in phospholipid bicelles.
Other aquaporins have also been successfully reconstituted in E. coli lipid membranes [27], [28].
AtmBAC1 was successfully reconstituted into artificial proteoliposomes and was able to carry out ornithine/ornithine exchange as well as exchange of various amino acids against arginine.
This approach is so far limited, in that cohesin loading onto DNA in a purified in vitro system has not yet been successfully reconstituted.
Whereas chanoclavine-I (2) has been successfully reconstituted in S. cerevisiae as well as another heterologous host, Aspergillus nidulans,[ 5, 11] more derivatized ergot alkaloids have not been subject to such efforts.
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