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Investigations were subsequently undertaken by MIT, Tufts, the OSI and the Congressional Subcommittee on Oversight and Investigations chaired by Rep. John D. Dingell (D-MI).
Modifications to the host BHK and CHO cells themselves were subsequently undertaken by including the anti-apoptotic gene Bcl-2 and a deletion mutant of Bcl-2 (Bcl-2Δ) as another method for limiting the cytopathic effects of the Sindbis virus.
Analyses were subsequently undertaken that identified complex chromosomal rearrangements, including deletions, translocations and ploidy differences between these cell lines [1].
Genetic studies were subsequently undertaken with [Brady]Rhizobium japonicum strains able to recycle H2 in Glycine max (soy) nodules [21], [22].
Primary and secondary antibody incubations were subsequently undertaken at 37°C for 1 hr each followed by nuclear staining using DAPI (1 µg/ml) diluted 1/500 in water.
Mock thefts were subsequently undertaken by a designated staff member at random times.
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A detailed design of the structures was subsequently undertaken and received positive acceptance from the stakeholders.
Molecular modeling of its docking within the hormone-binding domain of the receptor was subsequently undertaken.
Fuzzy k-means with extragrade (FKMe) classification was subsequently undertaken to account for Extragrades (i.e., outliers in the data).
A two-dimensional search was subsequently undertaken, whereby the selected overexpression targets were combined with the knock-out targets predicted by stoichiometric modeling.
Collective efforts toward further optimization of this novel tricyclic template to develop better porcupine inhibitors will be subsequently undertaken and reported in due course.
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