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Embryos were subsequently disaggregated in divalent cations-free M2 medium with careful and gentle pipetting.
Released crypts were subsequently disaggregated using 0·25% pancreatin (Sigma, St Louis, MO, USA) and the cell suspensions were stored on ice prior to use in experiments.
The monthly downscaled GCM time series from 2001 to 2099 were subsequently disaggregated into daily values by using monthly distributions of the daily 45 year ERA40 dataset from 1957 2002.
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Both were subsequently bailed.
"Both were subsequently released.
Five were subsequently released.
To address this issue, HBL cells were cultured in MCTS and subsequently disaggregated.
Spermatozoa from heterozygous and WT epididymides were released in clumps that subsequently disaggregated, forming a heterogeneous suspension of single and autoagglutinated spermatozoa.
Observed gauged daily amounts are interpolated to unsampled points and subsequently disaggregated using the sub-daily values obtained by the radar.
The tissue was treated with papain (10 units/ml) for 22 min at 37 °C, and subsequently disaggregated by repeated pipetting in DMEM/F-12 containing 10% FBS.
The epidermis was subsequently scraped from the dermis, minced with scalpels, and disaggregated by gentle pipetting.
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CEO of Professional Science Editing for Scientists @ prosciediting.com