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The hybrid F1 seeds were cultivated in 1946, and 3000 F2 individuals were subsequently cultivated at the Nagaoka National Agricultural Experiment Station in Niigata Prefecture; 65 promising individuals were selected from this generation.
An aliquot of 200 µL was separated (ex vivo sample) and the remaining parasites were subsequently cultivated in 0+ erythrocytes using a protocol adopted from Trager and Jensen [22].
Expanded cells were subsequently cultivated in pellets as described below.
Following treatment in an EGAS atmosphere, the cores were subsequently cultivated in an organotypic culture.
The cells were subsequently cultivated in modified Terrific Broth, consisting of 12 g tryptone, 24 g yeast extract, 2.31 g KH2PO4, and 12.54 g K2HPO4 per liter, supplemented with a 25 g/L carbon source for n-butanol production, but without added glycerol.
Similar(55)
To analyse further the viral effects on treated EGAS viability, another core of the same group was subsequently cultivated in organotypic culture as described in the following.
Both were subsequently bailed.
Five were subsequently released.
All were subsequently dropped.
Five were subsequently detained.
The females were subsequently released".
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