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Most of these artifacts were subsequently appropriated by the state via the Hebrew University of Jerusalem and its library.
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A large part of the coal so acquired by the Oil Corporation for use in its business was subsequently appropriated by it specifically to the use of the several vessels of the fleet and this use of the coal by vessels of the fleet was a use which had been contemplated by the parties when it was purchased.
The sections were subsequently stained using appropriate HRP or fluorochrome tagged secondary antibody and counterstaining were performed as described previously [69].
Cells and sections were subsequently incubated with appropriate fluorescently label-conjugated secondary antibodies (Molecular Probes, Invitrogen, Carlsbad, USA; Vector laboratories, Burlingame, USA).
The models were subsequently thinned as appropriate based on inspection of autocorrelation plots.
For double or triple immunolabeling, fixed cells were subsequently incubated with appropriate primary antibodies (Table 1) for 1 hour at room temperature.
The slides were subsequently treated with appropriate secondary antibodies conjugated to biotin, then developed utilizing avidin-conjugated horseradish peroxidase (HRP) with diaminobenzidine (DAB) as substrate (Vectastain ABC Kit from Vector Labs, Burlingame, CA).
Proposals were subsequently modified as appropriate after discussion with the SSC Convenor and their supervisor, in a series of iterations of educational objectives in terms of detail and focus.
For localization analysis of fusion proteins, the coding sequences were subsequently LR recombined to appropriate pUGT-DEST vectors.
The DSC and rheometric analysis results were subsequently used to define appropriate FFF process parameters.
The sections were subsequently treated with the appropriate biotinylated secondary antibodies (diluted 1∶200; Vector Laboratories, Burlingame, CA) and visualized with 0.01% diaminobenzidine tetrahydrochloride and 0.005% H2O2 in 50 mmol/L Tris HCl (pH 7.6).
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CEO of Professional Science Editing for Scientists @ prosciediting.com