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Thus, neither the newly written questions nor the assessment as a whole were subject to validation beyond internal review within the project team.
All the laboratory tests were subject to validation using National External Quality Assurance Schemes.
Therefore, 42 out of the 64 hippocampal and 27 out of the 36 prefrontal genes were subject to validation.
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Subsequently, 15 genes were subjected to validation by qRT-PCR.
These genes were subjected to validation by real-time PCR.
These apparent genomic imbalances were subjected to validation by independent methods.
All but one of these BAC clones could be recovered from the library and were subjected to validation studies.
Those 121 variants were subjected to validation by a direct Sanger sequencing, and 67 were confirmed to be present in the tumor, but absent from the normal samples.
All the protein sequences of these gene models were subjected to validation based on identification of homologues through BLASTP search against NRDB at E-value cutoff of 10−3.
Among the 40 genes that were subjected to validation, 33 (83 %) displayed the same trend observed in the microarray analysis of the six families (Additional file 3).
Many differentially regulated genes identified in the RNA-Seq analysis were subjected to validation through real-time qRT-PCR using GAPDH as a reference gene.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com