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If the incompleteness of reverse transcription and splicing were stringently considered, the total number of the genes corresponding to EST contigs was less than 7589, and at approximately 6100 minimum.
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To use so few crosses, the parents were stringently chosen (for methods see [ 10, 22]).
The localization results were stringently filtered for quality acceptance.
Prior to the addition of MutL, the free beads were stringently removed by extensive washing [17].
Raw reads were stringently demultiplexed by staff at the FAS Center for Systems Biology.
After the hybridization, slides were stringently washed as described in [ 73].
All ethical requirements including confidentiality of responses and informed consent were stringently ensured throughout the project.
The prediction and management applications were stringently validated in two stages.
Assembly parameters were stringently designed to assemble contigs representing sub-genomes within the allohexaploid genome.
In addition, the raw reads were stringently filtered before the de novo assembly.
The articles were stringently vetted and came out on top of a very big heap.
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