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Briefly, NB4PMN cells were stimulated for 1 min at 37 °C with or without 1 μM fMLP.
Constructs were stimulated for 14, 21 and 28 days respectively by different protocols, including cyclic mechanical stimulation, hormonal stimulation or a combination of both.
After extensive washing, cells were stimulated for indicated times.
Subsequently, cells were stimulated for 4 hours with LPS or Pam3Cys prepared in starvation medium.
Cells were stimulated for various periods with TNFα (Roche Biochemicals), glucose oxidase (Sigma), or H2O2 (Sigma).
Following the transfection, cells were stimulated for different times with ET-1 or cultured under hypoxia.
DC were stimulated for 4 24 hours with LPS, Pam3CSK4 or M. vaccae.
Melanophores were stimulated for aggregation or dispersion with 10 nM melatonin or 100 nM MSH, respectively.
Cells were stimulated for 72 hours and conditioned media were collected.
Following adherence the cells were stimulated for 30 min at 37°C.
Serum-starved cells were stimulated for 5 minutes with serum-free DMEM supplemented with recombinant human BDNF (Peprotech).
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