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In the prospective study, pimonidazole, CAIX and GLUT1 were stained on serial sections using single-staining procedures.
Finally, the grids were stained on drops of 0.4% Uranyl acetate and 1.8% methylcellulose for 8 min, picked up, and blotted.
As a control additional tissue sections were stained on the bench and treated in the same manner as those in the microwave.
(A) C2C12 cells were transfected 48 h in a differentiation medium, with a vector encoding collagen XXV fused to GFP (Col25a1-GFP) then live cells were stained on ice with an anti-GFP antibody.
Finally by D15, D cells were the only population in abundance that was remaining from the cells that were stained on D3, with only small numbers of EVP and TA observed.
Pollen viability: Pollen were harvested from 3 plants at random just before dehiscence and were stained on microscopic slide (200 300 grains counted per microscopic view and 3 views per slide) using 1% aqueous iodine-potassium iodide (I2KI) solution and analysed at 10× magnification using NIKON Alpha Shot-2 YS2H-E1112439 microscope.
Similar(10)
Embryos were stained on-slide as described (Duerr, 2006).
RAGE was stained on paraffin-embedded lung tissue.
My cookbook is stained on almost every page from use, and yours soon will be too.
Each tumour specimen was stained on ⩾3 separate occasions.
Enriched cells are stained on the filter for cytomorphological examination or further characterised by immunocytochemistry.
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