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While the number of violent episodes reported by administrators at Riverview had declined in recent months, the report issued last month said that there were "splits at all levels" that made it difficult to make changes at the hospital.
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As is standard practice in such trials, the women were split at random into two groups.
Following exposure to prions, cells were split at a ratio of 1 8 every three to four days.
K562 cultures were split at 2.5 × 105 cells per ml 1 day before transfection and were at ∼5 × 105 cells per ml when collected for transfection.
When necessary, they were split at 70% confluence.
hESC samples were split at various time points for karyotype analysis at the Children's Hospital Oakland Research Institute.
Occasionally stocks were split at the end of the first production year.
The medium was changed every 3 days, and the cultures were split at a 1∶8 ratio once every week.
Whole ears were split at the dermis-cartilage junction where the epidermis and dermis from the application side were retained for staining.
To evaluate whether cells can undergo self-renewal under this condition, they were split at a low density and grown for several passages with occasional freezing and thawing.
Statistically fewer (p<0.01) phESC colonies (38 colonies +-8) adhered after passaging when cells were split at the same plating density (50,000 cells/cm2) as conventionally-derived hESC (202 colonies +-36).
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CEO of Professional Science Editing for Scientists @ prosciediting.com