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In addition, peroxiredoxin 2, cofilin 1 and calreticulin were specifically recognised by sera from phenotypic subsets of patients with unidentified ANAs.
Our analysis revealed that PRDX2, cofilin 1 and calreticulin were specifically recognised by IgG from phenotypic subsets of patients with unidentified ANAs.
In sera proteolytically cleaved soluble L1 fragments as well as full-length L1 were specifically recognised by the monoclonal antibody UJ127, which was also used in the ELISA, underlining the specificity of the assay.
Fourteen and four proteins were recognised by IgG from at least 75% of the 15 pools in total and enriched nuclear protein extracts, respectively, whereas 15 protein spots were specifically recognised by IgG from at least four of the ten pools from patients with unidentified ANAs.
Finally, among the spots recognised by IgG from the 10 pools of sera from patients with unidentified ANAs, 15 were specifically recognised by IgG from at least 4 of these 10 pools in total or enriched nuclear protein extracts (Table 3) aSSc: systemic sclerosis.
In fact, western blotting of cell extracts from 22Rv1 cells synchronized at G2/M by treatment with nocodazole revealed several higher molecular weight proteins that were specifically recognised by anti-pSer GSK-3 antibodies, but not by anti-pTyr GSK-3 or total GSK-3 antibodies.
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These fragments are uniquely modified and thereby bear specific 'protein finger-prints', which are specifically recognised by markers developed by our group for the assessment of the remodelling of structural proteins that are involved in liver fibrosis.
Lomu was also inducted into the World Rugby Hall in October 2011, and was specifically recognised as one of four new inductees "who had left an indelible mark on Rugby World Cup for their moments of magic, inspiration or feats".
To find trypanosome proteins that are specifically recognised by sera from human sleeping sickness patients, we have screened the Trypanosoma brucei brucei proteome by Western blotting.
This analysis showed that the RpZRE3 core element is not recognised in its non-methylated state in the context of any of the viral or cellular promoters in the RpZRE3-promoter-18 data-set, and that this ZRE is specifically recognised when methylated.
The Sjda-His protein was specifically recognised by IgG in some infected mice as early as day 7 post-infection.
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