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Recovered cells were sorted to separate GFP + implanted MSC and host recruited GFP- cells.
Sex-sorted units were sorted to contain X chromosome-bearing sperm cells at an accuracy level of >90%.
Catches were sorted to species level or closest taxonomic level.
Twenty-four hours after transduction, transduction efficiency was measured by flow cytometry and cell samples with 30% positive cells were sorted to obtain a homogeneous population.
For detection of siRNA expression by Northern Blot, transduced MAGI-CCR-5 cells were sorted to over 98% EGFP-positive cells and total RNA was isolated using STAT-60 (Tel-Test, Friendswood, TX) reagent according to the manufacturer's recommendations.
Since DNA replication biases are partly visible at the dinucleotide level [30] [32], we have constructed individual codon-pair context maps in which rows and columns were sorted to separate P-site codons ending with a particular nucleotide (N3; rows) and A-site codons starting with a particular nucleotide (N1; columns) (Figure 4A).
Transcripts were sorted to identify thematic groupings across interviews.
Transcripts were sorted to identify putative biomarkers within the first 7 days following storage inception.
The 477 entries were sorted to identify autoantigens and viral/bacterial antigens, resulting in 377 potential functional genes, which were used for enrichment and pathway analysis.
Similar codes were sorted to form categories reflecting the manifest content of the text and similar categories were organized into themes reflecting the latent context of the text.
Harvested cells were sorted to obtain pure populations of the desired cell, before being subjected to DNA methylation analysis using microarrays and pyrosequencing.
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