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Approximately 3 × 10 tetrads were sorted for the initial cross on a FACSAria II (BD Bioscience), while 10 10 tetrads were sorted for subsequent backcrosses and intercrosses in a manner previously described (Ludlow et al. 2013; Scott et al. 2014).
Unfixed live cells were sorted four-ways using a FACS-Aria cell sorter instrument, and at least 1.5×106 cells were sorted for each subset of cells.
The 10,000 were sorted for each parameter and the 95% range determined from the sorted set.
The abundances of the top 35 genera were sorted for the analysis.
Vinyl, animal born, and debris were sorted for the shredding process.
Best1::GFP+ cells were sorted for qPCR analysis at day 35.
Thankfully, Hotel Lance was open and within minutes of the tweet going out, half a dozen competitors and their families were sorted for the night, free of charge.
Larvae were sorted for eGFP-RasV12 expression.
CD133+ cells were sorted for experiments.
YFP positive SP or non-SP cells were sorted for either Western analysis or functional assays.
Transduced cells were sorted for GFP expression and subsequently analyzed for Sulf-2 expression.
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