Sentence examples for were setup using from inspiring English sources

Using this dataset, ligand and protein structures were setup using two different methods, (i), calculating Gasteiger charges on both the ligands and the proteins using AutoDockTools and (ii), calculating PM6 charges on both the ligands and the proteins using MOPAC2009 [70] on Docking Server (see Method section for details).

Samples for quantitative RT-PCR were setup using the sybr green PCR master mix from Applied Biosystems (Foster City, CA) and primer pairs listed in table 1.

Explicit solvent orthogonal periodic boundary systems were setup using VMD and ions were added to a final equivalent concentration of 100 mM in order to neutralize the total charge of the systems.

Control binding experiments were setup using the LightShift EMSA optimization and control kit (Thermo).

Reactions were setup using an epMotion 5075 pipetting robot (Eppendorf, Hamburg, Germany) and amplifications were carried out in a LightCycler 480 (Roche).

All Taqman gene expression assays were setup using Taqman gene expression mastermix (Life Technologies, CA), and 75 ng of cDNA, from respective samples in 20 μl PCR reactions.

The discretization parameter (dx) was setup using 20 randomly selected targets (from a total of 40).

In order to examine the immune capabilities of fibroblasts in fish, an in vitro assay was setup using the rainbow trout RTHDF cell-line [53].

The server was setup using PostgreSQL (postgresql.org) and nginx/gunicorn (nginx.org; gunicorn.org).org

Treatment will be setup using reproducible positioning, verified using an on-line protocol, for all patients in this study.

The CMAQ v4.6 model was setup using the same vertical and horizontal grid structure as for WRF.