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Design points of experiments were set up using Taguchi experimental design method.
To assess the effects of malacidin on membrane depolarization, similar assays were set up using the membrane potential probe, DiBAC4 (bis- 1,3-dibutylbarbituric acid)trimethine oxonol).
Hanging or sitting drop crystallization trials were set up using commercial sparse-matrix screens (Rigaku TOP96 and Anatrace MCSG 1) and hits were subsequently refined.
Laboratory mesocosms were set up using tulip poplar (Liriodendron tulipifera) leaf litter and varying densities of earthworms in single and combined species treatments.
The experiments were set up using Minitab.
RT-qPCR reactions were set up using the 2× SYBR® Green JumpStart Taq Ready Mix (Sigma-Aldrich, Dorset, UK).
Reactive transport models were set up using characteristics of natural water systems (aquifers and rivers) in Bradford County, Pennsylvania.
Real-Time PCR assays were set up using an Eppendorf epMotion 5075 Robotics System (Eppendorf).
Reaction tubes were set up using the CAS1200 robotic system of Corbett Technologies.
Initial crystallization trials were set up using Crystal Screen I (Hampton) and MembFac (Nextal).
The crystallization trials were set up using an automated protein crystallization robot (Screenmaker 96+8, Innovadyne Technologies Inc. USA).
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