Temperature and rainfall were set to represent baseline and future conditions under the UKCP09 2080s high emissions scenario for July and August.
The lung voxels in the homogenous distribution (Table 1), containing values greater than half the maximum lung value, were set to represent the segmented lung.
In this analysis, pathogen parameter values were set to represent E. histolytica and E. dispar[ 56], with the major differences focused on virulence, infectivity, and infectiousness (See Table 1).
Different quadrants were set to represent the following populations of cells: quadrant 1 (Q1): live SYTO9-stained cells, quadrant 2 (Q2): double-stained membrane damaged cells, quadrant 3 (Q3): dead PI-stained cells and quadrant 4 (Q4): unstained cells.
The objective of the model (in silico cell) was growth and the constraints were set to represent the environmental conditions and the flux through the ammonium exchange reaction was calculated using linear optimization at steady state.
