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Cells treated with 1 μmol/L of DXMS alone were set as insulin resistant control while cells without any drug treatment were set as insulin sensitive control, respectively.
DXM-free 3T3-L1 adipocytes were set as insulin sensitive control.
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When direct data were missing but graft function was known to have been previously lost and not restored, insulin independence was set as dependent and C-peptide was set at 0. Otherwise, missing data were omitted (i.e., treated as missing at random) in the computations and modeling.
Fold stimulation refers to insulin secretion after various treatments compared to insulin secretion in 1 mM glucose treated cells, which was set as 1-fold.
Glucose consumed by cells of different treatments was expressed as relative glucose consumption over to insulin sensitive control cells whose relative glucose consumption was set as 100%[23]3].
As the system reverts to open-loop mode, the pump rate is set to deliver no insulin for 2 h.
dose of insulin solution as reference was set to 1 IU/kg.
As the IQWiG assessment of long-acting insulins analogues has not been finished yet, no reimbursement limit has been set for determir and glargine.
Nutrition may be turned off for other clinical reasons (0%) leaving only insulin as an intervention Same rules apply if parenteral nutrition is used ± 20% May be set to 0% if clinically specified.
The following parameters were also set as secondary endpoints: 2 h OGTT for glucose, insulin, proinsulin and C-peptide; AUC0 2h for glucose, insulin, proinsulin and C-peptide; the insulinogenic index 13 and body weight.
The hyperbolic relationship between insulin secretion and insulin sensitivity is characterized as insulin secretion = constant/insulin sensitivity.
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