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All amplicons were sequenced in parallel.
Each ID-tag was carefully designed so that it was easily distinguishable when up to four probes were sequenced in parallel during the validation reaction, without primer cross hybridization (Table 2 and Figure 6B).
Samples were sequenced in parallel in 6 runs.
Two independent biological replicates were sequenced in parallel.
The libraries were sequenced in parallel using a flow cell divided into 8 segments.
To validate ASA-PCR and HRM-PCR, the first 164 samples were sequenced in parallel, irrespective of genotyping results.
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Given a 250 bp amplicon and assuming 12,000 reads of ~250 bp length per 1/16th segment of a GS FLX sequencer picotiterplate (GS FLX amplicon protocol), 16 PCR products can be sequenced in parallel with 12,000× coverage.
To overcome the limitation in the number of samples that can be sequenced in parallel, the Multiplex Identifier was used.
This method of plastid enrichment therefore substantially increases the number of plastomes that can be sequenced in parallel.
However, "fusion primers" can become prohibitively expensive as the number of samples to be sequenced in parallel increases.
The Illumina 1 G Genome Analyzer (ILLUMINA, San Diego, CA, USA) uses a sequencing by synthesis method, during which millions of DNA fragments are sequenced in parallel (massive parallel sequencing).
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