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All the constructed plasmids were sequenced and analyzed on an ABI 3100 sequencer (Applied Biosystems).
The positive clones were sequenced and analyzed.
The amplified COI regions (710 bp) were sequenced and analyzed.
Of these, 1248 PCR positive clones were sequenced and analyzed.
The amplified catA genes (382 bp) were sequenced and analyzed.
Sixty eight SDIMO alpha subunit gene sequences derived from P Soil and seventy from Np Soil were sequenced and analyzed.
Cellulase gene detection screening showed that Cel genes was present in the MBAA3 and PCR products were sequenced and analyzed using the National center of biotechnological information nBlast database.
In our two trials, fecal samples from individual pigs were collected five times at 3-week intervals, and the 16S rRNA genes in the community DNAs from fecal samples were sequenced and analyzed.
Of the dozen subjects whose genomes were sequenced and analyzed, the team found a woman with no family history of breast cancer who had a mutation in the BRCA1 gene that makes her much more likely to develop the disease. .
At least five clones were sequenced and analyzed for each sample.
The amplified DNA was cloned using the TopoTA kit (Invitrogen), and individual clones were sequenced and analyzed for the presence of repeats (Table 1).
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