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The mice were sensitized on day 0, and on day 14, with intraperitoneal (i.p).
Briefly, mice were sensitized on day 0 by epicutaneous application onto the shaved abdomen of 25 µl of 0.5% DNFB (Sigma Aldrich, St. Quentin Fallavier, France) diluted in acetone/olive oil (4∶1, vol/vol) (i.e. vehicle).
Pigs were sensitized on study days 21, 35, and 49 by subcutaneous injection into the posterior section of the hind leg with 1.0 mg Ascaris suum allergen (ASA, Greer Laboratories, Lenoir, NC) plus alum (0.5 mL of 10% alum per 10 mg of protein) in 2 mL of saline.
Six- to 8-week old BALB/c male mice were sensitized on days 1 and 7 or 11 (see below) by intraperitoneal injection of 10 µg of ovalbumin (OVA Grade III; Sigma-Aldrich, Schnelldorf, Germany) emulsified in aluminum hydroxide (AlumInject; Perbio, Erembodegem, Belgium).
Refugee mothers were sensitized on the benefits of immunizing their children, a practice that was not common in South Sudan.
For elicitation mice were sensitized on the shaved abdomen with 200 μL 1% TNCB in acetone olive oil (4:1).
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Almost all our staff have not been sensitized on mental health and it is one of our missing links".
To conclude, the system cost and performance were important features, but dairy farmers should be sensitized on the consequences of lameness and its effect on farm profitability.
It was recommended that lecturers in the institutions of learning should be sensitized on the importance of students' perception of learning environment and enhanced student-student and student-lecturer relationships in their institutions.
After rinsing with methanol, PbS QDs are sensitized on TiO2 nanorods.
Farmers in Africa are also being sensitized on the internet of things and are conversely using these technologies to better their production and distribution capabilities.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com