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Genes from the 119 organisms were selected utilizing a "relaxed single-copy" approach, where inparalogs were initially allowed (all but one were removed from the final analyses) and genes were required to be present in only 95% of the organisms.
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Afterwards, the most preferred solution among all Pareto solutions is selected utilizing a fuzzy satisfying method.
Molecules were manually selected utilizing expert knowledge so they can be assigned to six clusters representing the original six combinatorial libraries the compounds were synthesized in.
Six separate candidate sequence sites of the AIE RNA binding protein gene were selected by utilizing the siRNA design support system R3 program (Takara Bio, Inc., Shiga, Japan) for constructing an optimal siRNA expression unit against the gene.
26 A total of 57 polymorphisms across nine genes, namely, ROCK2, MYLK, JNK1, TH, GNA11, GNB3, ADRA1A, ADRA1B, and ADRA1D, were selected by utilizing the ENSEMBL and HapMap databases along with related published literature for genotyping.
From 183 (63.7%) primer pairs that evidenced polymorphic alleles among the eight rose cultivars, 20 pairs evidencing EST sequence homology to known gene functions and high levels of polymorphism were selected and utilized for DNA fingerprinting and genetic diversity assessments of 47 rose hybrids.
Nine datasets, including three large-scale cancer datasets - breast cancer (BR) [27], multiple myeloma (MM) [28] and neuroblastoma (NB) [29] with six clinical endpoints contributed to the MAQC Consortium [24] and three datasets used in previously published prognostic modeling research [4], [25], [26], were selected and utilized in this study.
Cell-specific markers were selected and utilized in both immunostaining and quantification with RT-PCR.
For each sample, 18,808 effective sequences were selected and utilized to do the bacterial diversity and abundance analysis.
SNPs for which genotypes were called in at least 9 turkey populations (irrespective of whether SNPs were segregating in all these populations) were selected and utilized for the genetic diversity analysis.
Based on results from the preliminary sera screenings, IgG1, IgG2a and IgM were selected for evaluation utilizing the SouthernBiotech isotyping kit as described in the methods.
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