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On day 4 HDMEC were seeded upon the fibroblast monolayer.
Cells were seeded upon 80% confluence.
Caco-2 cells were seeded upon 10 mm coverslips coated with 20 μg/ml fibronectin in a 48-well plate at either low density or high density, and 2 h after seeding treated with the siRNA/transfection mix.
Data analysis was performed using GelDoc XR (BioRad, Berkeley, CA, USA). 1 × 10 Raw264.7 cells in 200 μL basic medium were seeded upon the 24-well Millicell Hanging Cell Culture Inserts (Millipore) with conditioned medium of ATF4 overexpressing tumor cells or control cells in the lower chamber.
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It employs a solid-phase, sequencing-by-synthesis method, where the DNA library, flanked by adapter sequences, is seeded upon on a lawn of oligonucleotides that coats the surface of the lanes on a flow cell.
"It's a product of where they were seeded based upon their competitive success," Mike Slive, chairman of the Division I men's basketball committee, said Friday in a telephone interview.
To evaluate macrophage morphology and cytoskeleton organization, 19×10 monocytes/cm were seeded on glass coverslips upon isolation, and left for 10 days in culture.
CV-1 cells were seeded onto coverslips, and upon reaching ∼70% confluency were transfected with the appropriate plasmids encoding Cerulean- and Venus-TF fusions, following the protocol supplied with Lipofectamine LTX reagent (Life Technologies).
Cells were seeded at equal densities and upon reaching confluence they were washed and incubated with heat-killed E.coli or control medium.
Upon transfection, cells were seeded at a density of 1000 cells per 10-cm dish and selected with G418 sulfate (2 mg/ml) for 2 weeks.
Transfected SKBR3 cells as wells as MCF-7 cells, upon Siah1 siRNA, were seeded at a density of 1 × 104 cells/well in a 96-well microplate and grown for 6 h and subsequently exposed to 0, 4 and 8 Gy IR.
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