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Then MG-63 cells were seeded on samples for cell proliferation and ALP activity tests.
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VSMCs were seeded on the samples with the density of 16,000 cells·cm−2 into 3 ml of Dulbecco's modified Eagle's minimum essential medium (Sigma, USA, cat. no. D5648), containing 10% fetal bovine serum (Sebak GmbH, Aidenbach, Germany).
L929 cells were seeded on top of the samples in the density of 30,000 cells per well in 1 mL of high glucose Dulbecco's modified Eagle's medium (DMEM, Sigma, USA) containing 10% fetal bovine serum (FBS, Invitrogen, USA) and 2 mM stable l-glutamine (l-alanyl-l-glutamine, Sigma, USA).
Cells (1 × 10) were seeded on poly-lysine-coated dishes, and samples incubated with serum-free RPMI-1640 at 37°C for 1 h to assure the complete adherence of cells to the poly-lysine-coated dishes.
Cells were seeded on a fixed surface area, 10 cm2 for arthritis samples and 75 cm2 for control samples.
For the in vitro angiogenesis assay samples, 10 cells were seeded on a thin layer of matrigel (BD, Franklin Lakes, USA) in a six well plate.
100 µl of each sample (∼1000 cells) were seeded on plates lacking uracil and either containing 2% glucose (no induction) or containing 2% galactose and 0.2% sucrose (induction).
The five samples submitted to culturing were seeded on sheep blood agar plates and incubated at 37 °C for 24 48 h.
HPMCs were seeded on glass coverslips.
HDFs and OSTs were seeded on substrates.
Chondrocytes were seeded on a confocal dish.
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