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Controls without matrigel were seeded in parallel.
For these determinations, primary cultures were seeded in parallel in 48 well plates (Corning Incorporated, NY), at a density of 2×104 cells/well in 500 µl of culture media.
Mouse embryonic fibroblasts (MEFs) were seeded in parallel into 24-well tissue culture plates at a density of 1×104 cells per well in full growth medium (DMEM plus 10% FBS).
Two chips were seeded in parallel whereby one was fixed and analyzed on day 1 (Fig. 7A) and the other chip was kept until day 3 (Fig. 7B) before analysis.
HEK 293 nontransformed cells were seeded in parallel.
For quantitative RT-PCR, 0.5x10 cells were seeded in parallel in 10 cm wells (N = 5).
Similar(51)
In parallel, cells were seeded in complete medium without LCA.
In parallel, cells were seeded in uncoated wells and treated similarly.
In parallel, cells were seeded in limiting dilutions in96-well plates (1, 3, 10 and 30 cells per well; 48 replica for eachcondition) to calculate the CFU-f frequency over subsequent passages.
In parallel, 10,000 pIL8-GFP A549 cells were seeded in 96-well microplates and stimulated with 20 ng/ml TNF-alpha and 30 μg/ml GC10 for 24 h.
In parallel, 0.17 × 106 cells were seeded in 25 cellculturelture flasks.
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