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Liquid samples were prepared at 1 w/v% in 1.5 mm quartz capillaries (Charles Supper), while gel samples were sectioned from tube samples and placed with aqueous solution in an aluminum three-well sample holder with kapton sides.
The crowns were sectioned from the roots with a diamond bur at the buccal cementoenamel junction, and each crown was cut longitudinally in an occlusogingival direction to obtain two buccal enamel surfaces.
Only three to five slices were sectioned from the surface of the resin block and the thickness of each section was less than 80 nm to make sure there were still enough samples for subsequent FIB-SEM experiments (Fig. 1D).
The hypothalami were sectioned from Bregma −1,6 to −4,2 mm.
The hypothalamus and the amygdala were sectioned from a 1 mm slice centered around Bregma −3.14 mm.
Horizontal or coronal slices (300 µm) were sectioned from the medial PFC by using a vibratome (DTK 1000 Zero 1, Microslicer, Japan).
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This electrolyte enables Nb to be sectioned from 1 70V (15–700 Å) and Ta to be sectioned from 1 100V (10 400 Å).
A plate of as-cast NiAl bronze (NAB) material was sectioned from a large casting.
To quantify tracheal submucosal gland sizes, serial sections were collected through the entire trachea, which was sectioned from dorsal to ventral aspects.
The tissue was sectioned from a block approximately 6 mm thick with the site of needle entry in the middle.
A 70 mm segment was sectioned from the base of leaf 4 of 14-day-old plants for superoxide determination.
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