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The tubes containing the mixture were rotated for 24 h in the dark and at room temperature (20°C) by a horizontal rotator at a fixed speed of 50 rpm (Denley Spiramix 5).
The resulting consensus tree was then visualized with Mega4 [ 43] and branches were rotated for legibility.
For each wash, tubes were rotated for 20 minutes at 4°C followed by brief centrifugation to pellet the Protein A/G agarose and bound complexes.
Tubes were rotated at 4 ° for 2 hr; 40 μL of Protein G Dynabeads or 40 μL of sheep α-mouse IgG Dynabeads (Invitrogen) were washed, resuspended in 1× meDIP buffer, and added to the 5-hmC and 5-mC IP, respectively, and tubes were rotated for 2.5 hr.
Players were rotated for different works, with roughly 150 performing in most pieces: the strength-in-numbers approach.
The lysates were rotated for 10 minutes at 4C, and spun for 30 minutes at 10,000g at 4C.
Similar(13)
The mixture was rotated for 5 hours at 4°C on an end to end rotator in a refrigerator to bind the antibody to Protein A agarose.
The converter is rotated for skimming the slag and pouring the blister copper.
The images are rotated for anonymity.
The figure is symmetrical because the position of the spiral with respect to the dipole is the same when it is rotated for positive and negative angles.
The cartilaginous supports of the epaulette shark's paired fins are reduced and separated when compared to other sharks, allowing them to be rotated for use as limbs.
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