Sentence examples for were restriction from inspiring English sources
noun
The act of restricting, or the state of being restricted.
Both the PCR fragment and the wild-type lenti-viral AR plasmid were restriction digested with Eco81I and Tth111I.
After PCR amplification, the fragments were restriction enzyme-digested and ligated into pEGFP-c2 vector (BD Biosciences) in frame with the EGFP sequence.
One microgram of genomic DNA extracted from either liver, testis, kidney or tail from independent mice were restriction digested with BglII and subjected to sodium bisulfite treatment as described [24].
Genomic DNA samples were restriction enzyme-digested and ligated with adaptors using the Invitrogen AFLP Analysis System II kit as described in the manual, with the exception that no denaturation was performed prior to ligation, as described by Bonin et al. [19].
These were restriction fragment length polymorphism (RFLP).
Following plasmid extraction, plasmids were restriction mapped using either EcoRV, EcoRI or BamHI (NEB).
Inevitably, though, there were restrictions.
All constructs used in this study were restriction-mapped and direct-sequenced (TaKaRa Biotech, Dalian, China) to confirm their authenticity.
Both plasmid pQlinkH and the PCR-amplified almF insert were restriction-digested, and plasmid DNA was treated with Antarctic phosphatase before ligation reactions were used to produce pQlinkHVc1578.
The remainder of proposals are restriction strategies.
