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The amplified ORF were restricted with HindIII and XbaI and cloned between the corresponding restriction sites of the mammalian expression vector pcDNA3.1.
The detected genes were confirmed by restriction analysis: sfaDE and papC amplicons (410 and 328 bp respectively) were restricted with HaeIII and yielded fragments of 209 bp, 135 bp and 66 bp; and 161 bp, 112 bp and 55 bp respectively.
"Their subscription unit generates a lot of cash," he said of Time Inc. "In the past, they were restricted with how to use that, and cash flows flowed to Time Warner.
He added: "For years, we were restricted with our finances.
The PCR product and pHT315 [41] were restricted with EcoRV and PvuII and the fragments ligated.
In brief, 0.5 µg DNA were restricted with RsaI and HinfI and resolved by constant field electrophoresis in a 0.8% agarose gel for 24 h at 50 V in TAE.
Similar(36)
(In fact, barely half of the area is restricted, with about ten per cent set aside for indigenous people).
Even apprenticeships could be restricted, with preference given to the sons of guild members or the sons of wealthy acquaintances.
For certain kinds of records, public access could be restricted, with archivists acting as guardians, he said.
Only proprietary trading is being restricted, with the banks having great (and perhaps too much) leeway to define what is proprietary and what is market-making.
In Arctic Canada glacier ice is restricted, with few exceptions, to the northeast as a consequence of the greater relief and precipitation around Baffin Bay and Davis Strait.
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