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Next, the liquid phase was carefully removed and biofilms were recovered into PBS and vortexed to resolve aggregated material.
In brief, either untreated cells or cells irradiated with 2 Gy gamma radiation were recovered into suspension by trypsinisation and washed twice in PBS.
Lipids retained in hemichannel preparations or in isolated plaques were recovered into a solution compatible for analysis by direct-infusion ESI-MS/MS.
To obtain a coding housekeeping gene phylogeny, the Burkholderiales core genes [ 81] were recovered into the clusters generated by comparative analysis among the fifteen Burkholderia species examined in study.
Cell suspensions in Tri-reagent were transferred to 1.7 ml low binding tubes (BioExcell; Worldwide Medical Products, Bristol, PA), incubated at 70°C for 10 min, centrifuged at 21,000 × g at 4°C for 10 min, and the supernatants were recovered into fresh tubes.
Frozen cell pellets were resuspended directly into 200 μl of Tri-reagent, heated at 60°C for 10 min, cleared of debris by centrifugation at 21,000 × g at 4°C for 10 min, and the supernatants were recovered into fresh tubes.
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The difference in both age and technique between the two sides becomes apparent as Toulouse rack up a daunting 312 runs in their 35 overs; many balls disappear, never to be recovered, into the surrounding vines.
The authors considered that enhancement of droplet deposition is induced downstream of the spacer where velocity profiles split by a spacer are recovered into one velocity profile; higher velocity in the core and lower velocity in a gap clearance between the rod surface and the spacer.
The results of this work suggest that alkaline-earth fission products accumulated in molten salts (Sr2+ and Ba2+) can be recovered into liquid Bi by electrochemical separation, which could be employed as a critical step for recycling the process salt (LiCl-KCl) in order to minimize the generation of additional nuclear wastes.
"Can't afford to retire," says Goldman, who adds he has put what share of the judgment he's recovered into the Ron Goldman Foundation for Justice that he founded with his wife and daughter. .
When a region of 337 bp in length was recovered into the 5'-UTR, the β-Galactosidase activity from pMP1cC fusion was 6-fold less than that from pMP1bC in the wild type M18, indicating that two negative control elements from +1 to +90 nt and from +255 to +337 nt were contained in the 5'-UTR of the phzA1-G1 transcript.
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