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(A) Full-length ATLs were reconstituted into proteoliposomes.
Single channels of Bacillus anthracis protective antigen, PA63, were reconstituted into planar lipid membranes and their inhibition by cationic aminopropylthio-β-cyclodextrin, AmPrβCD, was studied.
In brief, the full-length ATL proteins were reconstituted into the liposome, and the labeled donor proteoliposomes were mixed with unlabeled acceptor proteoliposomes in the presence of 5 mmol/L Mg2+ in a total volume of 100 µL per reaction.
Complexes of OprM and MexA, two proteins of the MexA MexB OprM multidrug efflux pump from Pseudomonasaeruginosa, an opportunistic Gram-negative bacterium, were reconstituted into proteoliposomes by detergent removal.
The wild-type YnaI and the mutant proteins were reconstituted into lipid vesicles composed of 1-palmitoyl-2-oleoyl-phosphatidylethanolamine (POPE, 7.5 mg/mL) and 1-palmitoyl-2-oleoyl-phosphatidylglycerol (POPG, 2.5 mg/mL) as previously described method (Li et al., 2007).
Purified aquaporin samples solubilized in different detergents were reconstituted into liposomes, and analyzed for the water channel activity.
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This image can be reconstituted into a paper cheque by the drawer's bank.
And indeed, here was a substance that could, like edible Plasticine, be reconstituted into brilliantly exciting shapes: Hula Hoops and French Fries and Pringles.
The plagioclase may be reconstituted into a more sodium-rich variety (albite), although the original form of the crystal is retained.
Messages (electronic messages, initially) were supposed to travel along this path, to be changed into electric energy by the transmitter, and to be reconstituted into intelligible language by the receiver.
Executives at Advance said the paper and its Web site, NOLA.com, would be reconstituted into a new company, the NOLA Media Group, as a way of adapting to the new ways news was being delivered and consumed in an increasingly digital age.
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