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Samples were reacted by individual acid addition (99%H3PO4at73 73 °C).
Lignin, resorcinol, and formaldehyde were reacted by polycondensation into a crosslinked phenolic resin network in the presence of colloidal poly methyl methacrylate) microspheres as the sacrificial template.
The mixed powders (weight ratio of W Ni Fe equals 93 4.9 2.1) were obtained by wet milling for 2 h, then the mixed powders with a weight of 2.0 g were reacted by holding for 6 h in a horizontal tube furnace at 800°C.
Parvalbumin sections were reacted by standard procedures, as described in Ichinohe and Rockland (2005).
As for a control, sections were reacted by replacing the first antibodies with non-immune mouse IgG or rabbit IgG.
For dual immunofluorescence staining of cultured CAF and NF cells grown in chamber slides, cells were fixed with 10% formalin for 10 min, followed by 0.2%Tritonx-100 for 10 min and blocked with 1% BSA/PBS, and slides were reacted by human α-SMA mouse monoclonal antibody and Alexa fluor 488-conjugated human IL-6 mouse monoclonal antibody sequentially as described above.
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During the CoLAVA process, the educt oxides are reacted by a gas phase condensation yielding amorphous spherical particles with diameters ranging from 10 to 100 nm.
Grafting was accomplished by reacting chitosan with acryloyl choride and the subsequent acrylamide grafted chitosan was reacted by a 1,2 intermolecular radical addition with BlocBuilder followed by NMP OEGMA/MEO2MA/AN monomers.
The pyridine nitrogen units on g-C3N4 backbone could absorb Ag+ through chemical absorption and then photo-generated electrons would be reacted by Ag+, leading to the inhibition of electrons transfer and decrement of photocurrent.
The nanotube (C60H24) and p-sulphonatocalix[4]arenes (C28H24O16S4) and fluorouracil (C4H3FN2O2) are reacted by Equations 1and 2, thus is formed products nanotube- fluorouracil (C64H26N2O2), p-sulphonatocalix[4]arenes- fluorouracil (C32H26N2O18S4) and HF in phases gas.
In M1, one series was reacted by immunofluorescence to enhance the CTB-Alexa488 signal, and in M2 and M3, this designated series was double reacted for CTB-gold by silver-enhancement.
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