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Kidneys were rapidly collected and snap frozen on dry ice.
Brains were rapidly collected and snap-frozen in cold isopentane.
Biopsy samples were rapidly collected at six to ten various locations from each placenta between the decidual and chorionic plates.
After sacrifice, brains were rapidly collected and frozen using isopentane and stored at −80°C until further processing.
Samples of the small and large bowel (n = 3 of each mouse line) of about 1 mm3 were rapidly collected and fixed in cold buffered Karnovsky's solution/2% glutardialdehyde/2% paraformaldehyde (pH 7.4) for 2 h.
After incubation for 3.0 h, the extracellular fluid was collected and the samples were processed to extract ADO as reported previously [28], [76]; briefly, the extracellular fluids were rapidly collected into microcentrifuge tubes and centrifuged at 14,000 x g for 1.0 min. Supernatants (400 µl) were placed into separate tubes and deproteinated with 20 µl of 100% trichloroacetic acid.
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Data to form and verify such models can be rapidly collected at the microwell-scale.
At the end of protocol, overnight-fasted rats were slightly anaesthetized with CO2 inhalation and blood was rapidly collected from sectioned carotid arteries and immediately transferred into a chilled tube of 6 ml containing 10.8 mg EDTA.
After the indicated times, animals were killed and the remnant liver was rapidly collected and frozen.
In conjunction with signs and symbols and an attached GPS, field data is rapidly collected.
Human metabolic waste products that are rapidly collected and pooled by the sewage systems can provide valuable evidence of the amount and type of any common product consumed by a population (Dove 2006).
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