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Libraries were quantitated with two different methods: Qubit fluorometer (Invitrogen) and quantitative PCR to ensure accuracy.
Moreover, roughly 1,900 of these proteins were quantitated with at least 2 unique peptides.
PCR products were quantitated with a gene-specific probe.
Supernatants containing the lysate were quantitated with Bradford protein assay (BioRad).
Blots were quantitated with the imaging and analysis software ImageJ 1.42d (http://rsbweb.nih.gov/ij/).
The amounts of GCase protein were quantitated with ImageQuant software and normalized to β-actin in the same samples.
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RNA was quantitated with RiboGreen RNA quantitation kits (Molecular Probes, Eugene, OR, USA), dissolved in diethylpyrocarbonate-treated H2O, and stored at -80°C until used.
RNA was quantitated with the RiboGreen RNA quantitation kit (Molecular Probes, Eugene, OR, USA), dissolved in diethylpyrocarbonate-treated H2O, and stored at -80°C until use.
Study design: CRP was quantitated with highly sensitive immunofluorometric (IFMA) and immunoenzymometric (IEMA) assays in 32 women with PPROM at 30.7±0.4 gestational weeks (mean±standard error of the mean) and in 27 gestational age-matched healthy women.
Band density was quantitated with ImageJ software.
CER was quantitated with the DGK assay [46].
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