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Sample extracts were quantified against an external standard prepared from 3R4F generated PM [17].
Sample extracts were quantified against an external standard prepared from filter extracted particulate matter (TPM) for the 3R4F reference or 1 mg cigarettes, as described previously [2, 9].
The samples were quantified against a heptadecanoic acid (17:0) internal standard, and the amount of each lipid species in the sample could, therefore, be expressed both as an absolute amount in the 0.1 g samples, and as a percentage of the total lipid present in the sample.
Peak areas were quantified against the added internal standard.
Samples were quantified against constructed UV and ESI-MS standard curves of compounds 4 and 8.
All experimental mRNA levels were quantified against the housekeeping gene hypoxanthine phosphoribosyltransferase (HPRT) as described previously [13].
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The surrogate models for SFR CFV-like cores performances are developed, biases and uncertainties are quantified against the CFV-v1 version.
The insulin concentration was quantified against a standard curved using absorbance at 450 nm (Wallac VICTOR plate reader 1420, Perkin Elmer, Waltham, MA, USA).
Purified protein was quantified against BSA standards by in-gel Coomassie Brilliant Blue total protein stain.
The amount of free acid released was quantified against standard curves.
If gametocytes are observed, they are quantified against 500 WBC.
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