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These gels cones were put into tissue capsules for the pre-treatment and following paraffin embedding.
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The tissue samples were diced in about 1 mm pieces that were put into 6-well tissue culture plates and fixed in the well under a cover slide.
Two hundred mg lyophilized ground samples of wild type and the mutant stem tissue were put into 10 ml ethanol: water (70 30 v/v) mix and samples were centrifuged at 14,000 rpm for 15 min at 4 °C.
Tissues were put into labelled cryo-tubes and immediately frozen in liquid nitrogen.
For mRNA levels, dissected tissues were put into RNA later (Roche).
The tissues were put into the sterile and frozen cryopreservation tubes and dipped into liquid nitrogen, and then conserved in -80°C ultra freezer until RNA extraction.
FFPE samples were cut to 10 μm thickness and two tissue slices were put into a 1.5 ml tube.
FFPE samples were cut to 10 μm thickness and several tissue slices were put into a 1.5 ml tube.
The normal- and tumor tissue samples were put into separate vials, weighed, and immediately thereafter 0.5 ml TRIzol (Life Technologies, Pailey, UK) was added.
Frozen tumour tissue specimens were put into ice-cold homogenising buffer (250 nmol l−1 sucrose, 10 mmol l−1 HEPES, 1 mmol l−1 EDTA, 1 mmol l−1 EGTA, 5 mmol l−1 DTT, 2 μg ml−1 aprotinin and 2 μg ml−1 leupeptin).
The knees were dissected to get cartilage tissues and then were put into RNAlator in order to isolate RNA for rtPCR.
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CEO of Professional Science Editing for Scientists @ prosciediting.com