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The proteins were purified by using a Ni-Sepharose column.
PCR products were purified by using the QIAquick Gel Extraction Kit (QIAGEN, Dusseldorf, Germany).
The CNTs and hybrids were purified by using a repetitive centrifugation process (three times), decanting the supernatant and using deionized H2O and 2-propanol to disperse them.
In brief, PCR products were purified by using gel extraction kit (Qiagen), and subsequently cloned into the pMD20 T vector (Transgen).
Mutant rhodopsins were purified by using 1D4-sepharose 4B affinity chromatography as previously described [24].
The wild type and mutants of the PDE9A2 catalytic domain were purified by using the similar protocols previously reported [24].
SCs were purified by using cytosine arabinoside and Thy-1.1 antibody (Sigma) mediated lysis of the fibroblasts.
After 2 4 weeks, primary cultures were purified by using modified immunopanning procedure described by Mi and Barres (1999) [68].
The PCR products were purified by using the PCR Purification Kit or the Gel Extraction Kit (Qiagen), depending on the degree of purity of the amplicons.
In PEG-induced reactions, the final products were purified by using 0.1M sodium bicarbonate buffer, pH 9.1 washing steps as previously described [29].
PCR products were purified by using QIAamp Gel Extraction Kit and were sequenced in the same way as for single cells.
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