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Phytophthora spp. isolates were provisionally identified from morphological features.
The remaining seven compounds were provisionally identified to be E-6,7-dihydroxydihydroligustilide(2), senkyunolide H 3), and senkyunolide I(4) [22] 22], coniferyl ferulate(5) [23] 23], senkyunolide A 6) [23] 23], E-ligustilide(8), and E-butylidenephalide(9) [23], respectively, by comparing their MS (accurate and tandem mass) and UV data with reported values.
Seedlings (= smallest leaf bearing stage, < 2 cm in length) and juveniles (plants > 2 cm in length, no anthesis) were provisionally identified on site by KMCC staff (Rajaovelona and Gardiner 2016) using subtle morphological features (e.g., presence of pseudobulbs) as well as proximity to mature plants on or near the same substrate.
These isolates, which were provisionally identified as EHDV, were sent to IAH Pirbright and passaged once in BHK-21 cells, then stored in the dsRNA-VRC as isolates ISR2006/01 to ISR2006/09 and ISR2006/13.
rRNA sequences were provisionally identified by BLAST query of 16S rRNA sequences extracted from the All-Species Living Tree Project database (version LTP_S95; [33]) and results corroborated through the RDP Classifier tool [34], [35], parsimony insertion into the SILVA SSURef_98 tree [36], and pairwise distance comparisons to aligned staphylococcal sequences.
ORFs of interest were provisionally identified using BLAST and PFAM.
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